Top: Typical image sequences (every 33 milliseconds) of a raft domain undergoing signaling (green) and a sphingomyelin analog (red). The sphingomyelin analog molecule was associated with a signaling raft domain from frame 4 to 9 (orange arrows). Bottom: Schematic model showing the transient recruitment of sphingomyelins to various raft domains in the time scale of 10 to 50 milliseconds. The signaling raft itself was stable for up to several tens of minutes, working as a stable signaling platform. Credit: Kyoto University iCeMS Researchers in Japan, India and France have found that molecules move into and out of a specialized region of the cell membrane, called the ’raft domain’, at unexpectedly fast rates. The discovery was made possible by developing fluorescent compounds that are structurally similar to a special class of lipids called sphingomyelins, and by using a home-built fluorescent microscope sensitive enough to detect single fluorescent molecules. Sphingomyelins are key molecules for the signalling functions and the formation of raft domains. But how they mediate the domains’ signalling functions was unknown.
A team of researchers led by Akihiro Kusumi and Kenichi Suzuki of Kyoto University’s Institute for Integrated Cell-Material Sciences (iCeMS) developed fluorescent compounds that are structurally similar to sphingomyelins and behave in a similar way. To make these fluorescent ’analogs’, they attached a hydrophilic (water-preferring) fluorescent molecule to the ’head’ part of synthetic sphingomyelin, with a hydrophilic linker group between them, without modifying a critical positive charge in the head group. This arrangement ensured that the fluorescent molecule was placed away from the interior of the cell membrane and did not interfere with the function of the sphingomyelin it was attached to or with the function of the cell membrane.
When the fluorescent sphingomyelin analogs were incorporated into the cell membrane of live mammalian cells in culture, they continually and very dynamically entered and exited from the cell membrane’s raft domains. Raft domains are extremely small, around 1/1,000th of the width of a human hair. But they occupy 10 to 20% of the cell membrane area. Quite unexpectedly, the sphingomyelins spent only 12 and 50 milliseconds inside the raft domain before and after extracellular immunostimulation respectively. For comparison, the eye blinks in about 400 milliseconds.
Discovering the very short residency times of sphingomyelins in raft domains represents a large paradigm shift in the research field of cell biology, molecular immunology and molecular neuroscience. Previously, most researchers in these fields believed that raft-associated lipids, including sphingomyelins, were stably localized in raft domains.
”The results suggest that the fluorescent sphingomyelin analogs developed here will be extremely useful for studying sphingomyelin interactions with many other raft-associated molecules and raft domains as well as for understanding the mechanisms of cell membrane signalling and of the invasion of various pathogens,” the researchers conclude.
Explore further:New fluorescent probes help solve cell membrane mystery
More information: Masanao Kinoshita et al, Raft-based sphingomyelin interactions revealed by new fluorescent sphingomyelin analogs, The Journal of Cell Biology (2017). DOI: 10.1083/jcb.201607086
Journal reference:Journal of Cell Biology
Provided by:Kyoto University